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Split-Plot Factorial Design

It is often inconvenient, costly, or even impossible to perform a factorial design in a completely randomized fashion. An alternative to a completely randomized design is a split-plot design. The use of split-plot designs started in agricultural experimentation, where experiments were carried out on different plots of land. Classical agricultural split-plot experimental designs were full factorial designs but run in a specific format. The key feature of split- plot designs is that levels of one or more factors are assigned to entire plots of land referred to as whole plots or main plots, whereas levels of other factors are assigned to parts of these whole or main plots. These parts are called subplots or split-plots. Split-plot designs thus have two types of experimental units, whole plots and subplots. The smaller experimental units, the subplots, are nested within the larger ones, the whole plots.

A Sample Design

Consider an experiment in which the effect of different types of nitrogen and grain varieties on the grain yield is investigated. Usually, it is convenient to treat entire plots of land (the whole plots) with the same type of nitrogen, whereas different grain varieties can typically be planted on small plots of land (the subplots) obtained by splitting each whole plot that was treated with one kind of nitrogen only. In this experimental setup, the first factor, the type of nitrogen, is called the whole-plot factor because its levels are applied to whole plots. The factor grain variety is called the subplot factor of the experiment because its levels are applied to the subplots. A schematic representation of a split- plot design involving type of nitrogen as the whole-plot factor and grain variety as the subplot factor is displayed in Figure 1. The four levels of the whole-plot factor are denoted by N1N4, whereas the three levels of the subplot factor are represented by G1G3. The split-plot design in this example has only one whole-plot factor and one subplot factor. Nevertheless, split-plot factorial designs with more than one whole-plot factor and more than one subplot factor can be constructed too. An example of a design with two whole-plot factors is given in the following discussion.

Design Layout

The layout of a split-plot design resembles that of a randomized block design. The key difference between split-plot designs and randomized block designs is that, in randomized block designs, the factor level combinations are randomly assigned to the experimental units in the blocks. In split-plot designs, a completely random assignment is impossible because all the subplots within one whole plot must be treated with the same level of the whole-plot factor(s). Because of this restriction on the assignment of factor-level combinations to the plots, only a restricted form of randomization is possible. The restricted randomization is carried out in two steps. First, the whole-plot factor-level combinations are randomly assigned to the whole plots. Next, the subplot factor-level combinations are randomly assigned to the subplots within each whole plot. This is done so that each subplot factor-level combination appears once in every whole plot.

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