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Researchers often assess the internal validity of a study’s research design. An assessment of internal validity is an evaluation of the ability of a research design to provide evidence of a cause-and-effect relationship between an independent variable and a dependent variable. Often, researchers seek to conclude that a program or a treatment led to a difference in a dependent variable, but to come to that conclusion, researchers must rule out many other potential causal factors. A researcher may present a logical argument about the possible causal relationship between an independent and a dependent variable that are correlated, but absent adequate internal validity, conclusions may be questioned and alternative explanations for the relationship put forward.

Before a researcher begins a study, the researcher must consider the research design and take steps to avoid or take into consideration threats to internal validity. Threats to internal validity can be thought of as plausible alternative explanations for a study’s findings. Donald T. Campbell and Julian C. Stanley’s 1963 study and Thomas T. Cook and Campbell’s 1979 study identified and described several general categories of events, which, if not controlled for through research design, can lead to threats to a study’s internal validity.

This entry discusses threats to internal validity and steps that can be taken to reduce threats to internal validity through choices in research design. Examples of alternative explanations for causal relationships are provided to illustrate the threats to internal validity.

The general categories of threats to internal validity include the following: history, maturation, testing, instrumentation, mortality (or subject attrition), regression to the mean, selection, diffusion or imitation of treatments, compensation, compensatory rivalry, and demoralization.

History

History, or events outside of a research study, can influence subjects’ responses and can act as unexpected and unplanned independent variables. For example, a campaign to reduce cigarette smoking among teenagers may coincide with a decrease in cigarette purchases by teens, but an increased tax on cigarettes during the same time period may also be associated with a reduction in cigarette purchases. The “event” of increasing taxes on cigarettes may be an alternative explanation for the reduction in cigarette purchases.

A researcher may attempt to control for history threats by making all participants’ experiences identical except for the independent variables, but this is often difficult or impossible to do. Other ways to reduce the threat to internal validity due to history is to engage in random assignment and to include a control group in the research design. This ideally results in a research study in which the effects of history will be similar across groups.

Longitudinal studies or studies involving repeated measures on subjects over time are more susceptible to threats to internal validity due to history than studies in which data are collected at a single point in time.

Maturation

Maturation is another common threat to internal validity in longitudinal studies. Maturation refers to the natural changes, psychological or physiological, that occur as participants age or time passes. Studies that involve children are often most vulnerable to maturation effects. For example, perhaps you have implemented a behavioral therapy among a group of 3-year-olds. When one measures their behavior at age 4 years, these children demonstrate remarkable improvement. The question a researcher must ask is whether the program led to improvements in behavior, or whether the children are more well-behaved because they have naturally become more mature. Maturation threats to internal validity can also be related to physiological changes. For example, participants may become hungrier, thirstier, or more tired as a study drags on, and hunger, thirst, or fatigue (but not your independent variable) may lead participants to respond differently on measures of the dependent variable.

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