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The Bonferroni technique is used to hold a familywise Type I error rate to a preselected value when a family of related SIGNIFICANCE TESTS (or confidence intervals) is conducted. This situation, sometimes called MULTIPLE COMPARISONS, or, more generally, “simultaneous inference,” may arise when statistical tests are carried out on data from the same units (e.g., when people respond to several questionnaire items or when correlations among several variables are calculated on the same people), or when statistical estimates such as means or proportions are used more than once in a set of hypotheses (e.g., when a set of planned or post hoc comparisons are not statistically independent). Although the Bonferroni technique could be applied when the tests are independent, its use in such cases is extremely rare.

The Bonferroni technique has several advantages that make it an attractive alternative to other simultaneous inference procedures. Among these are that it may be applied to any statistical test resulting in a PVALUE, that it is very easy to use, and that it does not result in much wasted power when compared with other simultaneous significance testing procedures.

Development

The basis of the Bonferroni technique is Bonferroni's inequality: αf ≤ α1 + α2 + ··· + αm. Because it is αf that the researcher wishes to control, then choosing values for the several αj terms that make α1 + α2 +···+ αm = αj will result in control over αf, the familywise Type I error rate. Usually, the several αj values are set equal to each other, and each test is run at αt = αf/m. The value of αf is therefore, at most, mαt.

Application

In order to apply the Bonferroni technique, we must have a family (i.e., set) of m (at least two) significance tests and will define two significance (α) levels: the per-test Type I error rate and the familywise Type I error rate. The per-test Type I error rate, αt, is the significance level of each statistical test (i.e., the null hypothesis is rejected if the p value of the test falls below αt). The familywise Type I error rate, αf, is the probability that one or more of the m tests in the family is declared significant as a Type I error (i.e., when its null hypothesis is true).

Use of the Bonferroni technique involves adjusting the per-test Type I error rate such that αt = αf/m. In practice, the familywise Type I error rate is usually held to .05. Thus, αt is usually set at .05/m.

Although tables of these unusual percentiles of various distributions (e.g., t, chi-square) exist, in practice, the Bonferroni technique is most easily applied using the p values that are standard output in statistical packages. The p value of each statistical test is compared with the calculated αt and if p ≤ αt, the result is declared statistically significant; otherwise, it is not.

Example

A questionnaire with four items has been distributed to a group of participants who have also been identified by gender. The intent is to test, for each item, whether there is a difference between the distributions of males and females. CHI-SQUARE TESTS based on the four two-way tables (gender by item response) will be used. However, if each test were conducted at the α = .05 level, the probability of at least one of them reaching statistical significance by chance (assuming all null hypotheses true) is greater than .05 because there are four opportunities for a Type I error, not just one. In this example, there are m = 4 significance tests, so instead of comparing the p value for each test with .05, it is compared with .05/4 = .0125.

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