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Honestly Significant Difference (HSD) Test
When an analysis of variance (ANOVA) gives a significant result, this indicates that at least one group differs from the other groups. Yet, the omnibus test does not inform on the pattern of differences between the means. To analyze the pattern of difference between means, the ANOVA is often followed by specific comparisons, and the most commonly used involves comparing two means (the so-called pairwise comparisons).
An easy and frequently used pairwise comparison technique was developed by John Tukey under the name of the honestly significant difference (HSD) test. The main idea of the HSD is to compute the honestly significant difference (i.e., the HSD) between two means using a statistical distribution defined by Student and called the q distribution. This distribution gives the exact sampling distribution of the largest difference between a set of means originating from the same population. All pairwise differences are evaluated using the same sampling distribution used for the largest difference. This makes the HSD approach quite conservative.
Notations
The data to be analyzed comprise A groups; a given group is denoted a. The number of observations of the a. The group is denoted Sa. If all groups have the same size, it is denoted S. The total number of observations is denoted N. The mean of Group a is denoted Ma+. Obtained from a preliminary ANOVA, the error source (i.e., within group) is denoted S(A), the effect (i.e., between group) is denoted A. The mean square of error is denoted MSS(A) and the mean square of effect is denoted MS A.
Least Significant Difference
The rationale behind the HSD technique comes from the observation that, when the null hypothesis is true, the value of the q statistics evaluating the difference between Groups a and a′ is equal to

and follows a Studentized range q distribution with a range of A and N − A degrees of freedom. The ratio t would therefore be declared significant at a given α level if the value of q is larger than the critical value for the α level obtained from the q distribution and denoted qA, α where v = N − A is the number of degrees of freedom of the error, and A is the range (i.e., the number of groups). This value can be obtained from a table of the Studentized range distribution. Rewriting Equation 1 shows that a difference between the means of Group a and a′ will be significant if

When there is an equal number of observation per group, Equation 2 can be simplified as

To evaluate the difference between the means of Groups a and a′, the absolute value of the difference between the means is taken and compared with the value of HSD. If then the comparison is declared significant at the chosen a level (usually .05 or .01). Then this procedure is repeated or all





Note that HSD has less power than almost all other post hoc comparison methods (e.g., Fisher's LSD or Newmann—Keuls) except the Scheffe approach and the Bonferonni method because the α level for each difference between means is set at the same level as the largest difference.
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