Profiling Study Methods

Stem cells pertain to undifferentiated, pluripotent cells that are capable of self-renewal. Technically, stem cells are categorized according to their source, which is either an embryo or adult tissue. Embryonic stem cells are produced from preimplantation embryos, specifically from the inner cell mass of a blastocyst- or morula-stage embryo. Blastomeres, as well as primordial germ cells, are also sources of embryonic stem cells. Various profiling methods have been employed to fully characterize stem cells, including the molecular and genetics mechansims that control and maintain their capacity for pluripotency. These methodologies have also assisted in improving methods of generating and manipulating stem cells for biomedical applications.