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Mouse ES Cell Isolation

MOUSE EMBRYONIC STEM (ES) cells are pluripo—tent and have the ability to divide into any cell types that form an organism. They are derived from early embryos before they implant into the uterus (blastocysts). Blastocysts are made of an outer layer (trophectoderm) that aids implantation and eventually forms the placenta, and an inner cell mass that will give rise to the germ layers and the organism. ES cells are produced by removing the inner cell mass of a blastocyst. After isolation, the blastocyst is no longer able to complete implantation and thus is destroyed.

Characteristics

Researchers have come up with a list of criteria that ES cells must fulfill. ES cells must have normal chromosomes, meaning that they have the number and length of chromosomes of its ...

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